The Fox1 ferroxidase of Chlamydomonas reinhardtii: a new multicopper oxidase structural paradigm
نویسندگان
چکیده
منابع مشابه
The involvement of a multicopper oxidase in iron uptake by the green algae Chlamydomonas reinhardtii.
In the unicellular green algae Chlamydomonas reinhardtii, high-affinity uptake of iron (Fe) requires an Fe(3+)-chelate reductase and an Fe transporter. Neither of these proteins nor their corresponding genes have been isolated. We previously identified, by analysis of differentially expressed plasma membrane proteins, an approximately 150-kD protein whose synthesis was induced under conditions ...
متن کاملA putative protein kinase Femu9p contributes to the iron deficiency-inducible expression of FOX1 gene in Chlamydomonas reinhardtii
FOX1 gene encoding a multicopper ferroxidase is vital in iron metabolism in the single-celled green algae Chlamydomonas reinhardtii. Molecular characteristics of this gene have been extensively studied. However, little information is available about its regulatory factors in C. reinhardtii. In this study, we isolated a regulatory mutant of FOX1 gene by using an arylsulfatase gene driven by the ...
متن کاملCopper-dependent iron assimilation pathway in the model photosynthetic eukaryote Chlamydomonas reinhardtii.
The unicellular green alga Chlamydomonas reinhardtii is a valuable model for studying metal metabolism in a photosynthetic background. A search of the Chlamydomonas expressed sequence tag database led to the identification of several components that form a copper-dependent iron assimilation pathway related to the high-affinity iron uptake pathway defined originally for Saccharomyces cerevisiae....
متن کاملA C2H2 Zinc Finger Protein FEMU2 Is Required for fox1 Expression in Chlamydomonas reinhardtii
Chlamydomonas reinhardtii fox1 gene encodes a ferroxidase that is involved in cellular Fe uptake and highly induced during Fe deficient conditions. In an effort to identify fox1 promoter regulatory elements, an insertional library was generated in a transgenic Chlamydomonas strain (2A38) harboring an arylsulfatase (ARS) reporter gene driven by the fox1 promoter. Mutants with a defective respons...
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ژورنال
عنوان ژورنال: JBIC Journal of Biological Inorganic Chemistry
سال: 2008
ISSN: 0949-8257,1432-1327
DOI: 10.1007/s00775-008-0450-z